Roland Cy-5 Dual Trigger Cymbal Pad, 10In

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Kaur, G., Lewis, J.S. & van Oijen, A.M. Shining a Spotlight on DNA: Single-Molecule Methods to Visualise DNA. Molecules 24 (2019). Cyanine dyes are used to label proteins, antibodies, peptides, nucleic acid probes, and any kind of other biomolecules to be used in a variety of fluorescence detection techniques: Flow cytometry, Microscopy (mainly Visible range, but also UV, IR), Microplate assays, Microarrays, as well as "light-up Probes," and in vivo imaging. [18] Nucleic acid labeling [ edit ] Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms

a b Mujumdar B, Ernst A, Mujumdar SR, Lewis CJ, Waggoner AS (Mar 1993). "Cyanine dye labeling reagents: Sulfoindocyanine succinimidyl esters". Bioconjugate Chemistry. 4 (2): 105–111. doi: 10.1021/bc00020a001. PMID 7873641. where two quaternary nitrogens are joined by a polymethine chain. [5] Both nitrogens may each be independently part of a heteroaromatic moiety, such as pyrrole, imidazole, thiazole, pyridine, quinoline, indole, benzothiazole, etc. CY-2 (长缨-2) is a development of CY-1, and it is based on C-802 missile, sharing the same turbo jet engine. The missile is in limited service in Chinese navy after numerous tests, the last of which was the test of the air-launched version, which was successfully completed by Harbin SH-5 in 1994. The major improvement is the range of this weapon is tripled to 30 nautical miles (56km), but the speed is reduced to subsonic level from CY-1's Mach 1.5. Just like the way CY-1 can be stored and launched from the container/launcher of C-801, CY-2 can be stored and launched from the container/launcher of C-802. The publicized data for CY-2 includes: Harvey, B. J., Perez, C. & Levitus, M. DNA sequence-dependent enhancement of Cy3 fluorescence. Photochem. Photobiol. Sci. 8, 1105–1110 (2009).Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed The amount of Cy5.5 required for reaction depends on the amount of protein to be labeled, and the optimal molar ratio of Cy5.5 to protein is about 10.

The following protocol is an example of dye-protein conjugate purification by using a SepHadex G-25 column. The excitation source will now appear on the graph display as a thin vertical line in a color associated with its location on the spectrum. Harvey, B. J. & Levitus, M. Nucleobase-specific enhancement of Cy3 fluorescence. J. Fluoresc. 19, 443–448 (2009). Agbavwe, C. et al. Efficiency, error and yield in light-directed maskless synthesis of DNA microarrays. J. Nanobiotechnol. 9 (2011). Cy3B: This product is manufactured under an exclusive license from Carnegie Mellon University and is covered by US patent number 6,133,445 and equivalent patents and patent applications in other countries.are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, Cy5.5) = mmol (Cy5.5) ×MW (Cy5.5) / mg/μL (Cy5.5) = 6.7 ×10-5 mmol ×753.88 mg/mmol / 0.01 mg/μL = 5.05 μL (Cy5.5) Doktycz, M. J., Paner, T. M., Amaratunga, M. & Benight, A. S. Thermodynamic stability of the 5’ dangling-ended DNA hairpins formed from sequences 5’-(Xy)2ggatac(T)4gtatcc-3’, Where X, Y = a, T, G, C. Biopolymers 30, 829–845 (1990). Mujumdar, R. B., Ernst, L. A., Mujumdar, S. R., Lewis, C. J. & Waggoner, A. S. Cyanine dye labeling reagents—sulfoindocyanine Succinimidyl Esters. Bioconjugate Chem. 4, 105–111 (1993).

Add more PBS (pH 7.2-7.4) to the desired sample to complete the column purification. Combine the fractions that contain the desired dye-protein conjugate.Norman, D. G., Grainger, R. J., Uhrin, D. & Lilley, D. M. J. Location of cyanine-3 on double-stranded DNA: Importance for fluorescence resonance energy transfer studies. Biochemistry 39, 6317–6324 (2000). any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any Among currently available fluorescent dyes, the cyanine dyes are better able to withstand the harsh dehydration and embedding conditions required for mounting sections in non-polar plastic media, such as DPX and Permount™. The cyanine dyes are brighter in the non-polar environment than in an aqueous medium, resulting in less acquisition time in the confocal microscope than that required for DyLight and Alexa Fluor® dyes (Figure 1), even though those dyes are brighter in aqueous mounting media. Perez-Gonzalez, C., Lafontaine, D.A. & Penedo, J.C. Fluorescence-Based Strategies to Investigate the Structure and Dynamics of Aptamer-Ligand Complexes. Front. Chem. 4 (2016). Turner, D. H., Sugimoto, N. & Freier, S. M. Rna structure prediction. Annu. Rev. Biophys. Bio 17, 167–192 (1988).